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Peptide phage display 기술을 이용한 나노입자의 materials recognition 응용 -part II : Fe3O4 나노입자를 이용한 magnetic bio-panningApplication of Nanoparticles for Materials recognition Using Peptide Phage Display Technique - Part II: Magnetic Bio-panning Using Fe3O4 Nanoparticles
- Other Titles
- Application of Nanoparticles for Materials recognition Using Peptide Phage Display Technique - Part II: Magnetic Bio-panning Using Fe3O4 Nanoparticles
- Authors
- 이창우; 김민정; Standaert, Robert F.; 김세연; Owens, Elizabeth T.; Yan, Jun; 좌용호; Doktycz, Mitchel J.; 이재성
- Issue Date
- Mar-2008
- Publisher
- 대한금속·재료학회
- Keywords
- Fe3O4 nanoparticles; magnetic bio-panning; phage display; histidine; materials recognition
- Citation
- Korean Journal of Metal and Materials, v.46, no.3, pp 131 - 134
- Pages
- 4
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Korean Journal of Metal and Materials
- Volume
- 46
- Number
- 3
- Start Page
- 131
- End Page
- 134
- ISSN
- 1738-8228
2288-8241
- Abstract
- The magnetism of Fe3O4 nanoparticles was applied to magnetic bio-panning process for finding specific sequences against Fe3O4 crystal phase. Vibrating sample magnetometer (VSM) measurement showed that the coercivity of 30 Oe and the saturation magnetization of 55 emu/g were sufficient in controlling particle movement and magnetizing particles in the media, respectively. This ferrimagnetism of nanoparticles practically enhanced panning efficiency by exaggerating centrifuge step and preventing particle loss. Sequencing results showed that histidine which was commonly found in peptide sequences played an important role in the binding onto Fe3O4 nanoparticle surface. However, various possible motifs were also observed from several neighboring amino acids of histidine.
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Related Researcher
- CHOA, YONG HO
- ERICA 첨단융합대학 (ERICA 신소재·반도체공학전공)