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마이크로 혼합기와 반응기로 구성된 DNA 결찰용 바이오칩에 관한 연구A study about biochip combined with micro mixer and reactor for DNA ligation
- Other Titles
- A study about biochip combined with micro mixer and reactor for DNA ligation
- Authors
- Kang, Do-Hyoung; Ahn, Yoomin; Hwang, Seung Yong
- Issue Date
- Aug-2008
- Publisher
- 대한기계학회
- Keywords
- DNA ligation; Microbiochip; Micromixer; PVP coating
- Citation
- Transactions of the KSME, A, v.32, no.8, pp 624 - 632
- Pages
- 9
- Indexed
- SCOPUS
KCI
- Journal Title
- Transactions of the KSME, A
- Volume
- 32
- Number
- 8
- Start Page
- 624
- End Page
- 632
- ISSN
- 1226-4873
2288-5226
- Abstract
- In this research, we developed new PDMS-glass based microbiochip consisted of the micromixer and microreactor for DNA ligation. The micromixer was composed of a straight channel integrated with nozzles and pillars, and the microreactor was composed of a serpentine channel. We coated the PDMS chip surface with the 0.25wt.% PVP solution to prevent the bubble generation which was caused by the hydrophobicity of the PDMS. The new micomixer was passive type and the mixing was enhanced by a convective diffusion using the nozzle and pillar. The 10.33mm long micromixer showed the good mixing efficiency of 87.7% at 500 □ 1/min flow rate. We could perform the DNA ligation successfully in the microbiochip, and the ligation time was shortened from 4 hours in conventional laboratory method to 5 min in the microbiochip.
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Related Researcher
- Hwang, Seung Yong
- ERICA 첨단융합대학 (ERICA 분자의약전공)