Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Dong Woo | - |
dc.contributor.author | Nettles, Dana L. | - |
dc.contributor.author | Setton, Lori A. | - |
dc.contributor.author | Chilkoti, Ashutosh | - |
dc.date.accessioned | 2021-06-23T19:41:22Z | - |
dc.date.available | 2021-06-23T19:41:22Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.issn | 1525-7797 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43761 | - |
dc.description.abstract | In situ gelation of injectable polypeptide-based materials is attractive for minimally invasive in vivo implantation of biomaterials and tissue engineering scaffolds. We demonstrate that chemically cross-linked elastin-like polypeptide (ELP) hydrogels can be rapidly formed in aqueous solution by reacting lysine-containing ELPs with an organophosphorous cross-linker, beta-[tris(hydroxymethyl)phosphino]propionic acid (THPP) under physiological conditions. The mechanical properties of the cross-linked ELP hydrogels were largely modulated by the molar concentration of lysine residues in the ELP and the pH at which the cross-linking reaction was carried out. Fibroblasts embedded in ELP hydrogels survived the cross-linking process and were viable after in vitro culture for 3 days. DNA quantification of ELP hydrogels with encapsulated fibroblasts indicated that there was no significant difference in DNA content between day 0 and day 3 when ELP hydrogels were formed with an equimolar ratio of THPP and lysine residues of the ELPs. These results suggest that THPP cross-linking may be a biocompatible strategy for the in situ formation of cross-linked hydrogels. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dong Woo | - |
dc.identifier.doi | 10.1021/bm061059m | - |
dc.identifier.scopusid | 2-s2.0-34249867928 | - |
dc.identifier.wosid | 000246413600013 | - |
dc.identifier.bibliographicCitation | BIOMACROMOLECULES, v.8, no.5, pp.1463 - 1470 | - |
dc.relation.isPartOf | BIOMACROMOLECULES | - |
dc.citation.title | BIOMACROMOLECULES | - |
dc.citation.volume | 8 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1463 | - |
dc.citation.endPage | 1470 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | PROTEIN-BASED POLYMERS | - |
dc.subject.keywordPlus | INVERSE TEMPERATURE TRANSITION | - |
dc.subject.keywordPlus | CARTILAGINOUS TISSUE-REPAIR | - |
dc.subject.keywordPlus | TARGETED DRUG-DELIVERY | - |
dc.subject.keywordPlus | MECHANICAL-BEHAVIOR | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | THERMOPLASTIC ELASTOMERS | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | MOLECULAR-WEIGHT | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/bm061059m | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
55 Hanyangdeahak-ro, Sangnok-gu, Ansan, Gyeonggi-do, 15588, Korea+82-31-400-4269 sweetbrain@hanyang.ac.kr
COPYRIGHT © 2021 HANYANG UNIVERSITY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.