Rapid cross-linking of elastin-like polypeptides with (hydroxymethyl)phosphines in aqueous solution
- Authors
- Lim, Dong Woo; Nettles, Dana L.; Setton, Lori A.; Chilkoti, Ashutosh
- Issue Date
- May-2007
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOMACROMOLECULES, v.8, no.5, pp.1463 - 1470
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMACROMOLECULES
- Volume
- 8
- Number
- 5
- Start Page
- 1463
- End Page
- 1470
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43761
- DOI
- 10.1021/bm061059m
- ISSN
- 1525-7797
- Abstract
- In situ gelation of injectable polypeptide-based materials is attractive for minimally invasive in vivo implantation of biomaterials and tissue engineering scaffolds. We demonstrate that chemically cross-linked elastin-like polypeptide (ELP) hydrogels can be rapidly formed in aqueous solution by reacting lysine-containing ELPs with an organophosphorous cross-linker, beta-[tris(hydroxymethyl)phosphino]propionic acid (THPP) under physiological conditions. The mechanical properties of the cross-linked ELP hydrogels were largely modulated by the molar concentration of lysine residues in the ELP and the pH at which the cross-linking reaction was carried out. Fibroblasts embedded in ELP hydrogels survived the cross-linking process and were viable after in vitro culture for 3 days. DNA quantification of ELP hydrogels with encapsulated fibroblasts indicated that there was no significant difference in DNA content between day 0 and day 3 when ELP hydrogels were formed with an equimolar ratio of THPP and lysine residues of the ELPs. These results suggest that THPP cross-linking may be a biocompatible strategy for the in situ formation of cross-linked hydrogels.
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