Suppression of the NF-kappa B signalling pathway by ergolide, sesquiterpene lactone, in HeLa cells
- Authors
- Chun, Jae Kwang; Seo, Dong-Wan; Ahn, Seong Hoon; Park, Jae Hyun; You, Jueng-Soo; Lee, Chang-Hee; Lee, Jae Cheol; Kim, Yong Kee; Han, Jeung-Whan
- Issue Date
- Apr-2007
- Publisher
- Pharmaceutical Press
- Citation
- Journal of Pharmacy and Pharmacology, v.59, no.4, pp.561 - 566
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Pharmacy and Pharmacology
- Volume
- 59
- Number
- 4
- Start Page
- 561
- End Page
- 566
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43800
- DOI
- 10.1211/jpp.59.4.0011
- ISSN
- 0022-3573
- Abstract
- We have previously reported that ergolide, a sesquiterpene lactone isolated from Inula britannica, suppresses inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression by inhibiting nuclear factor-kappa B (NF-kappa B) in RAW 264.7 macrophages. In this study, we show that ergolide suppresses the DNA binding activity of NF-kappa B and nuclear translocation of NF-kappa B p65 subunit, leading to the inhibition of NF-kappa B-dependent gene transcription in 12-O-tetradecanoylphorbol 13-acetate (TPA)-stimulated HeLa cells. We also show that ergolide decreases the degradation and phosphorylation of I kappa B, an inhibitory protein of NF-kappa B, and this effect is accompanied by a simultaneous reduction of I kappa B kinase (IKK) activity. However, ergolide does not inhibit in-vitro IKK activity directly, suggesting the possible involvement of upstream IKK kinases in the regulation of NF-kappa B activation. Furthermore, ergolide-mediated protein kinase C alpha (PKC alpha) inhibition is involved in reduction of NF-kappa B inhibition, as demonstrated by the observation that dominant negative PKC alpha, but not p44/42 MAPK and p38 MAPK, inhibits TPA-stimulated reporter gene expression. Taken together, our results suggest that ergolide suppresses NF-kappa B activation through the inhibition of PKC alpha-IKK activity, providing insight for PKC alpha as a molecular target for anti-inflammatory drugs.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43800)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.