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Preparative isolation and purification of geniposide from gardenia fruits by centrifugal partition chromatography

Authors
Kim, Chul YoungKim, Jinwoong
Issue Date
Mar-2007
Publisher
John Wiley & Sons Inc.
Keywords
centrifugal partition chromatography; iridoid glycoside; geniposide; Gardenia jasminoides; Rubiaceae
Citation
Phytochemical Analysis, v.18, no. 2, pp 115 - 117
Pages
3
Indexed
SCIE
SCOPUS
Journal Title
Phytochemical Analysis
Volume
18
Number
2
Start Page
115
End Page
117
URI
https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/43835
DOI
10.1002/pca.958
ISSN
0958-0344
1099-1565
Abstract
The iridoid glycoside, geniposide was purified by centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate:isopropanol:water (3:2:5, v/v) from an 80% methanolic extract of fruits of Gardenia jusminoides. Preparative CPC yielded 56.2 mg of geniposide in a one-step separation of 500 mg of extract, with a purity of 95% as determined by HPLC. Isolated gemposide was identified from its H-1-NMR, C-13-NMR and MS spectra. Copyright.
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