Pretreatment of corn stover by soaking in aqueous ammonia at moderate temperatures
- Authors
- Kim, Tae Hyun; Lee, Y. Y.
- Issue Date
- Apr-2007
- Publisher
- HUMANA PRESS INC
- Keywords
- biofuel; bioethanol; biomass conversion; simultaneous saccharification and cofermentation; hemicellulose; lignin
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.137, pp.81 - 92
- Indexed
- SCIE
SCOPUS
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 137
- Start Page
- 81
- End Page
- 92
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44299
- DOI
- 10.1007/s12010-007-9041-7
- ISSN
- 0273-2289
- Abstract
- Soaking in aqueous ammonia at moderate temperatures was investigated as a method of pretreatment for enzymatic hydrolysis as well as simultaneous saccharification and cofermentation (SSCF) of corn stover. The method involves batch treatment of the feedstock with aqueous ammonia (15-30 wt%) at 40-90 degrees C for 6-24 h. The optimum treatment conditions were found to be 15 wt% of NH3, 60 degrees C, 1 : 6 of solid-to-liquid ratio, and 12 h of treatment time. The treated corn stover retained 100% glucan and 85% of xylan, but removed 62% of lignin. The enzymatic digestibility of the glucan content increased from 17 to 85% with 15 FPU/g-glucan enzyme loading, whereas the digestibility of the xylan content increased to 78%. The treated corn stover was also subjected to SSCF test using Spezyme-CP and recombinant Escherichia coli (KO11). The SSCF of the soaking in aqueous ammonia treated corn stover resulted in an ethanol concentration of 19.2 g/L from 3% (w/v) glucan loading, which corresponds to 77% of the maximum theoretical yield based on glucan and xylan.
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