Glass-polydimethysiloxane hybrid microthermostat for restriction enzyme digestion
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, Yong-Jun | - |
dc.contributor.author | Kim, Dae Jin | - |
dc.contributor.author | Cho, Woong | - |
dc.contributor.author | Ahn, Yoomin | - |
dc.contributor.author | Hwang, Seung-Yong | - |
dc.date.accessioned | 2021-06-23T20:42:37Z | - |
dc.date.available | 2021-06-23T20:42:37Z | - |
dc.date.issued | 2007-01 | - |
dc.identifier.issn | 0255-5476 | - |
dc.identifier.issn | 1662-9752 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44309 | - |
dc.description.abstract | This paper reports a low-cost microthermostat that is able to maintain a constant temperature necessary for restriction enzyme digestion. Polydimethylsiloxanc (PDMS) and Pyrex glass were used to make the microthermostat, because PDMS is a cheap and mass-producible material and both PDMS and glass have very good biocompatibility compared to the more commonly used silicon, A heater made of Au wiring patterned on Pyrex glass was used to control the temperature. A PDMS replica molding technique was used to fabricate a reaction chamber with 3.6 mu l capacity. Restriction enzyme digestion was performed by using the fabricated microthermostat and by a conventional method. Then, using gel electrophoresis, we compared results between the microthermostat and conventional methods. It was found that restriction enzyme digestion using the microthermostat required 5 min of heating. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Trans Tech Publications Ltd. | - |
dc.title | Glass-polydimethysiloxane hybrid microthermostat for restriction enzyme digestion | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.4028/www.scientific.net/MSF.544-545.335 | - |
dc.identifier.scopusid | 2-s2.0-38349115466 | - |
dc.identifier.wosid | 000246224300084 | - |
dc.identifier.bibliographicCitation | Materials Science Forum, v.544-545, pp 335 - 338 | - |
dc.citation.title | Materials Science Forum | - |
dc.citation.volume | 544-545 | - |
dc.citation.startPage | 335 | - |
dc.citation.endPage | 338 | - |
dc.type.docType | Proceedings Paper | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Environmental | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordAuthor | glass-PDMS chip | - |
dc.subject.keywordAuthor | microthermostat | - |
dc.subject.keywordAuthor | restriction enzyme digestion | - |
dc.subject.keywordAuthor | DNA chip assay | - |
dc.identifier.url | https://www.scientific.net/MSF.544-545.335 | - |
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