Glass-polydimethysiloxane hybrid microthermostat for restriction enzyme digestion
- Authors
- Ko, Yong-Jun; Kim, Dae Jin; Cho, Woong; Ahn, Yoomin; Hwang, Seung-Yong
- Issue Date
- Jan-2007
- Publisher
- Trans Tech Publications Ltd.
- Keywords
- glass-PDMS chip; microthermostat; restriction enzyme digestion; DNA chip assay
- Citation
- Materials Science Forum, v.544-545, pp 335 - 338
- Pages
- 4
- Indexed
- SCIE
SCOPUS
- Journal Title
- Materials Science Forum
- Volume
- 544-545
- Start Page
- 335
- End Page
- 338
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44309
- DOI
- 10.4028/www.scientific.net/MSF.544-545.335
- ISSN
- 0255-5476
1662-9752
- Abstract
- This paper reports a low-cost microthermostat that is able to maintain a constant temperature necessary for restriction enzyme digestion. Polydimethylsiloxanc (PDMS) and Pyrex glass were used to make the microthermostat, because PDMS is a cheap and mass-producible material and both PDMS and glass have very good biocompatibility compared to the more commonly used silicon, A heater made of Au wiring patterned on Pyrex glass was used to control the temperature. A PDMS replica molding technique was used to fabricate a reaction chamber with 3.6 mu l capacity. Restriction enzyme digestion was performed by using the fabricated microthermostat and by a conventional method. Then, using gel electrophoresis, we compared results between the microthermostat and conventional methods. It was found that restriction enzyme digestion using the microthermostat required 5 min of heating.
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Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
- COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MECHANICAL ENGINEERING > 1. Journal Articles
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