Liquid chromatography-tandem mass spectrometric determination of ceramides and related lipid species in cellular extracts
- Authors
- Yoo, Hye Hyun; Son, Junghyun; Kim, Dong-Hyun
- Issue Date
- Nov-2006
- Publisher
- Elsevier BV
- Keywords
- sphingolipid; ceramides LC-MS/MS; HL-60 cells
- Citation
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, v.843, no.2, pp.327 - 333
- Indexed
- SCOPUS
- Journal Title
- Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
- Volume
- 843
- Number
- 2
- Start Page
- 327
- End Page
- 333
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44495
- DOI
- 10.1016/j.jchromb.2006.06.025
- ISSN
- 1570-0232
- Abstract
- A liquid chromatography-electrospray ionization tandem mass spectrometric (LC-MS/MS) method was developed for the simultaneous qualitative and quantitative determination of sphingolipid metabolites such as ceramides, sphingisine, sphinganine, sphingomyelins, and ceramide 1-phosphates in the extracts of human promyelocytic leukemia cells (HL-60). The assay uses C-4 ceramide as an internal standard; simple liquid extraction; a short XTerra MS C-18 (3 mu m, 50 mm x 2.0 mm) column; a gradient mobile phase of 5 mM ammonium formate (pH 4.0)/methanol/tetrahydrofuran (5/2/3 -> 1/2/7); mass spectrometric detection using electrospray ionization. This LC-MS/MS method allowed the identification of 22 sphingolipid derivatives at pmol levels. In addition, this technique was successfully applied to analyze the changes of the sphingolipids profiles in cancer cells treated with apoptosis inducing agents, C-2 ceramide and H2O2. (c) 2006 Elsevier B.V. All rights reserved.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF PHARMACY > DEPARTMENT OF PHARMACY > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.