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Preparative isolation of mangiferin from Anemarrhena asphodeloides rhizomes by centrifugal partition chromatography
- Authors
- Young Kim, Chul; Ahn, Mi-Jeong; Kim, Jinwoong
- Issue Date
- Jun-2006
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- centrifugal partition chromatography; Anemarrhena asphodeloides; preparative chromatography; mangiferin; xanthone glycoside
- Citation
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.29, no.6, pp.869 - 875
- Indexed
- SCOPUS
- Volume
- 29
- Number
- 6
- Start Page
- 869
- End Page
- 875
- ISSN
- 1082-6076
- Abstract
- Mangiferin was separated from rhizomes of Anemarrhena asphodeloides extract using centrifugal partition chromatography (CPC) with a two-phase solvent system composed of ethyl acetate-isopropanol-water (3:2:5, v/v). Mangiferin (22.5 mg) was successfully isolated from methanolic extracts (957 mg) in only one step, and the purity of isolated compound was determined to be over 95% by HPLC analysis. The structure of mangiferin was identified by H-1, C-13 NMR and ESI-MS spectral data analysis.
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Related Researcher
- Kim, Chul Young
- COLLEGE OF PHARMACY (DEPARTMENT OF PHARMACY)