Inhibition of cell-cycle progression in HeLa cells by HY52, a novel cyclin-dependent kinase inhibitor isolated from Bauhinia forficata
- Authors
- Lim, Haeyoung; Kim, Min Kyoung; Lim, Yoongho; Cho, Youl-Hee; Lee, Chul-Hoon
- Issue Date
- Feb-2006
- Publisher
- Elsevier BV
- Keywords
- HY52; Bauhinia forficata; Pata de Vaca; cell-cycle arrest
- Citation
- Cancer Letters, v.233, no.1, pp 89 - 97
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- Cancer Letters
- Volume
- 233
- Number
- 1
- Start Page
- 89
- End Page
- 97
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/45036
- DOI
- 10.1016/j.canlet.2005.03.002
- ISSN
- 0304-3835
1872-7980
- Abstract
- In the course of screening for a novel inhibitor of cyclin-dependent kinase (CDK), HY52 (C17H30O2N2; molecular weight 294) was isolated from the leaves of Pata de Vaca (Bauhinia foificata). The growth of HeLa cells was inhibited in a dose-dependent manner when treated with 0.07 to 0.41 mM of HY52 for 24 h (IC50: 0.11 mM). Furthermore, HY52 showed the selective inhibitory activity oil CDC2 kinase purified using immunoprecipitation with all IC50 value of 0.45 mM. A flow cytometric analysis of the HeLa cells treated with HY52 revealed an,in appreciable cell-cycle arrest ill the G I phase. Moreover, a TUNEL assay exhibited the apoptotic induction of HeLa cells treated with HY52. To obtain further information on the cell-cycle arrest induced by HY52 the expression of certain cell-cycle-associated proteins was examined using a Western blot analysis. The results revealed that HY52 was found to inhibit the proliferation of HeLa cells through inducing a G1-phase arrest by inhibiting pRb phosphorylation via all up-regulation of p21(WAF1/CIPI) and p27(KIPI), and G2/M-phase arrest by down-regulation of CDC2, cyclin A, and cyclin B1.
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