유산균을 이용한 경구용 항원 단백질 수송능 연구

Abstract

A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS (“generally regarded as safe”) status, we tested whether L. lactis could function as the carrier of the L1 protein of human papillomavirus (HPV) type 16. The RNA level expression of L1 gene was detected in L. Lactis. The L1 protein was expressed in L. lactis with L1 gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with L1-encoding plasmid (pAMJ328-L1) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L. lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that L1 protein did not affect the biochemical property of L. lactis. These results suggest that L. lactis, capable of carrying L1 protein, might be further developed as a biocompatible oral protein delivery system.