HPLC determination of dexamethasone in human plasma
- Authors
- Yun-Kyoung Song; Jeong-Sook Park; Jin-Ki Kim; Chong-Kook Kim
- Issue Date
- Aug-2004
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- dexamethasone; HPLC; human plasma; bioavailability
- Citation
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, v.27, no.14, pp.2293 - 2306
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
- Volume
- 27
- Number
- 14
- Start Page
- 2293
- End Page
- 2306
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46570
- DOI
- 10.1081/JLC-200025726
- ISSN
- 1082-6076
- Abstract
- A sensitive high performance liquid chromatography (HPLC) method has been developed for the determination of dexamethasone in human plasma. After 1.5 mL of plasma was extracted with 4 mL of ethyl acetate containing 80 ng/mL of desoxymethasone, analysis of dexamethasone in plasma samples was carried out using a Sphereclone ODS2 column with UV detection for separation and quantification. A mixture of acetonitrile-10 mM phosphate buffer (pH 7.0) (32:68, v/v) was used as a mobile phase. The limit of quantitative (LOQ) analysis was 10 ng/mL. The accuracy of the assay was from 96.96% to 106.07%, while the intra- and inter-day coefficient of variation of the same concentration range was less than 15%, except LOQ (<20%). The signals were monitored by a UV detector at 240 bn with flow-rate of 1.0 mL/min. The method could be applied, with great success, to evaluate the bioavailability of dexamethasone in human subjects, with excellent selectivity and reproducibility and clinical study.
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