Enhancement of polyethylene glycol (PEG)-modified cationic liposome-mediated gene deliveries: effects on serum stability and transfection efficiency
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin-Ki | - |
dc.contributor.author | Choi, Sung-Hee | - |
dc.contributor.author | Kim, Cheong-Ok | - |
dc.contributor.author | Park, Jeong-Sook | - |
dc.contributor.author | Ahn, Woong-Shick | - |
dc.contributor.author | Kim, Chong-Kook | - |
dc.date.accessioned | 2021-06-24T00:44:00Z | - |
dc.date.available | 2021-06-24T00:44:00Z | - |
dc.date.created | 2021-01-21 | - |
dc.date.issued | 2003-04 | - |
dc.identifier.issn | 0022-3573 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46706 | - |
dc.description.abstract | In this study, we modified cationic liposomes either by polyethylene glycol (PEG)-grafting or PEG-adding methods, and compared the physical properties of transfection complexes and transfection efficiency in-vitro and prolonged circulation in-vivo. The PEG-grafted transfection complexes were prepared by mixing plasmid DNA with PEG-grafted cationic liposomes, which were composed of DSPE-PEG 2000 and cationic lipids. The PEG-added transfection complexes were prepared by adding DSPE-PEG 2000 to the mixture of cationic liposomes and plasmid DNA. The particle sizes of the PEG-modified transfection complexes (similar to200nm) changed a little over 4 weeks compared with the conventional transfection complexes. In the presence of serum, the transfection efficiency of the conventional transfection complexes was lowered whereas the transfection efficiency of the PEG-modified transfection complexes was maintained. Moreover, the transfection efficiency of the conventional transfection complexes was significantly reduced when they were stored. However, the transfection efficiency was stable for the PEG-modified transfection complexes, even after two weeks of storage. Of the in-vitro transfection efficiencies, there was no difference between PEG-grafted and PEG-added transfection complexes. When the conventional, PEG-grafted, and PEG-added transfection complexes were administered into mice by the tail vein, the PEG-added transfection complexes showed a prolonged circulation of plasmid DNA compared with other transfection complexes. These results suggest that the PEG-added transfection complexes could be a useful non-viral vector because of their simplicity in preparation, enhanced stability and prolonged circulation compared with the conventional transfection complexes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL PHARMACEUTICAL SOC GREAT BRITAIN | - |
dc.title | Enhancement of polyethylene glycol (PEG)-modified cationic liposome-mediated gene deliveries: effects on serum stability and transfection efficiency | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jin-Ki | - |
dc.identifier.doi | 10.1211/002235702928 | - |
dc.identifier.scopusid | 2-s2.0-0038399864 | - |
dc.identifier.wosid | 000183281600005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PHARMACY AND PHARMACOLOGY, v.55, no.4, pp.453 - 460 | - |
dc.relation.isPartOf | JOURNAL OF PHARMACY AND PHARMACOLOGY | - |
dc.citation.title | JOURNAL OF PHARMACY AND PHARMACOLOGY | - |
dc.citation.volume | 55 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 453 | - |
dc.citation.endPage | 460 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | LIPID FORMULATIONS | - |
dc.subject.keywordPlus | DNA COMPLEXES | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | TUMORS | - |
dc.subject.keywordPlus | PHARMACOKINETICS | - |
dc.subject.keywordPlus | NANOSPHERES | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | OXIDE | - |
dc.identifier.url | https://academic.oup.com/jpp/article/55/4/453/6148253 | - |
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