Enhancement of polyethylene glycol (PEG)-modified cationic liposome-mediated gene deliveries: effects on serum stability and transfection efficiency
- Authors
- Kim, Jin-Ki; Choi, Sung-Hee; Kim, Cheong-Ok; Park, Jeong-Sook; Ahn, Woong-Shick; Kim, Chong-Kook
- Issue Date
- Apr-2003
- Publisher
- ROYAL PHARMACEUTICAL SOC GREAT BRITAIN
- Citation
- JOURNAL OF PHARMACY AND PHARMACOLOGY, v.55, no.4, pp.453 - 460
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF PHARMACY AND PHARMACOLOGY
- Volume
- 55
- Number
- 4
- Start Page
- 453
- End Page
- 460
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46706
- DOI
- 10.1211/002235702928
- ISSN
- 0022-3573
- Abstract
- In this study, we modified cationic liposomes either by polyethylene glycol (PEG)-grafting or PEG-adding methods, and compared the physical properties of transfection complexes and transfection efficiency in-vitro and prolonged circulation in-vivo. The PEG-grafted transfection complexes were prepared by mixing plasmid DNA with PEG-grafted cationic liposomes, which were composed of DSPE-PEG 2000 and cationic lipids. The PEG-added transfection complexes were prepared by adding DSPE-PEG 2000 to the mixture of cationic liposomes and plasmid DNA. The particle sizes of the PEG-modified transfection complexes (similar to200nm) changed a little over 4 weeks compared with the conventional transfection complexes. In the presence of serum, the transfection efficiency of the conventional transfection complexes was lowered whereas the transfection efficiency of the PEG-modified transfection complexes was maintained. Moreover, the transfection efficiency of the conventional transfection complexes was significantly reduced when they were stored. However, the transfection efficiency was stable for the PEG-modified transfection complexes, even after two weeks of storage. Of the in-vitro transfection efficiencies, there was no difference between PEG-grafted and PEG-added transfection complexes. When the conventional, PEG-grafted, and PEG-added transfection complexes were administered into mice by the tail vein, the PEG-added transfection complexes showed a prolonged circulation of plasmid DNA compared with other transfection complexes. These results suggest that the PEG-added transfection complexes could be a useful non-viral vector because of their simplicity in preparation, enhanced stability and prolonged circulation compared with the conventional transfection complexes.
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