Microencapsulation of recombinant Saccharomyces cerevisiae cells with invertase activity in liquid-core alginate capsules
DC Field | Value | Language |
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dc.contributor.author | Chang, Ho Nam | - |
dc.contributor.author | Seong, Gi Hun | - |
dc.contributor.author | Yoo, Ik-Keun | - |
dc.contributor.author | Park, Joong Kon | - |
dc.contributor.author | Seo, Jin-Ho | - |
dc.date.accessioned | 2021-06-24T01:09:37Z | - |
dc.date.available | 2021-06-24T01:09:37Z | - |
dc.date.issued | 1996-07 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.issn | 1097-0290 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/47052 | - |
dc.description.abstract | As a means of integrating cell growth and immobilization, recombinant Saccharomyces cerevisiae cells with invertase activity were immobilized in liquid-core alginate capsules and cultured to a high density. S. cerevisiae cells of SEY 2102 (MAT alpha ura3-52 leu2-3, 112 his4-519) harboring plasmid pRB58 with the SUC2 gene coding for invertase were grown to 83 g/L of liquid-core volume inside the capsule on a dry weight basis. The cloned invertase was expressed well in the immobilized cells with slightly higher activity than the free cells in a batch culture. Invertase in the immobilized cells showed slightly more improved thermal stability than in the free cells. Storage in a Na-acetate buffer at 4 degrees C and 10 degrees C for 1 month resulted in 7% and 8% loss in activity, respectively. The sucrose hydrolysis reaction was stably maintained for 25 repeated batches for 7 days at 30 degrees C. Continuous hydrolysis of 0.3 M sucrose was carried out in a packed bed reactor with a conversion of more than 90% at a maximum productivity of 55.5 g glucose/L per hour for 7 days. In a continuous stirred tank reactor, the maximum productivity of 80.8 g glucose/L per hour was achieved at a conversion of 59.1% using 1.0 M sucrose solution, and 0.5 M sucrose solution was hydrolyzed for 1 week with a 95% conversion at a productivity of 48.8 g/L per hour. (C) 1996 John Wiley & Sons, Inc. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Microencapsulation of recombinant Saccharomyces cerevisiae cells with invertase activity in liquid-core alginate capsules | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1002/(SICI)1097-0290(19960720)51:2<157::AID-BIT4>3.0.CO;2-I | - |
dc.identifier.scopusid | 2-s2.0-15844405967 | - |
dc.identifier.wosid | A1996UR42000004 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.51, no.2, pp 157 - 162 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 51 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 157 | - |
dc.citation.endPage | 162 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | YEAST-CELLS | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | MEMBRANE | - |
dc.subject.keywordAuthor | calcium-alginate capsules | - |
dc.subject.keywordAuthor | microencapsulation | - |
dc.subject.keywordAuthor | invertase | - |
dc.subject.keywordAuthor | recombinant Saccharomyces cerevisiae | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/abs/10.1002%2F%28SICI%291097-0290%2819960720%2951%3A2%3C157%3A%3AAID-BIT4%3E3.0.CO%3B2-I? | - |
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