Microencapsulation of recombinant Saccharomyces cerevisiae cells with invertase activity in liquid-core alginate capsules
- Authors
- Chang, Ho Nam; Seong, Gi Hun; Yoo, Ik-Keun; Park, Joong Kon; Seo, Jin-Ho
- Issue Date
- Jul-1996
- Publisher
- WILEY-BLACKWELL
- Keywords
- calcium-alginate capsules; microencapsulation; invertase; recombinant Saccharomyces cerevisiae
- Citation
- BIOTECHNOLOGY AND BIOENGINEERING, v.51, no.2, pp 157 - 162
- Pages
- 6
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOTECHNOLOGY AND BIOENGINEERING
- Volume
- 51
- Number
- 2
- Start Page
- 157
- End Page
- 162
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/47052
- DOI
- 10.1002/(SICI)1097-0290(19960720)51:2<157::AID-BIT4>3.0.CO;2-I
- ISSN
- 0006-3592
1097-0290
- Abstract
- As a means of integrating cell growth and immobilization, recombinant Saccharomyces cerevisiae cells with invertase activity were immobilized in liquid-core alginate capsules and cultured to a high density. S. cerevisiae cells of SEY 2102 (MAT alpha ura3-52 leu2-3, 112 his4-519) harboring plasmid pRB58 with the SUC2 gene coding for invertase were grown to 83 g/L of liquid-core volume inside the capsule on a dry weight basis. The cloned invertase was expressed well in the immobilized cells with slightly higher activity than the free cells in a batch culture. Invertase in the immobilized cells showed slightly more improved thermal stability than in the free cells. Storage in a Na-acetate buffer at 4 degrees C and 10 degrees C for 1 month resulted in 7% and 8% loss in activity, respectively. The sucrose hydrolysis reaction was stably maintained for 25 repeated batches for 7 days at 30 degrees C. Continuous hydrolysis of 0.3 M sucrose was carried out in a packed bed reactor with a conversion of more than 90% at a maximum productivity of 55.5 g glucose/L per hour for 7 days. In a continuous stirred tank reactor, the maximum productivity of 80.8 g glucose/L per hour was achieved at a conversion of 59.1% using 1.0 M sucrose solution, and 0.5 M sucrose solution was hydrolyzed for 1 week with a 95% conversion at a productivity of 48.8 g/L per hour. (C) 1996 John Wiley & Sons, Inc.
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Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
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