Two-stage processing of Miscanthus giganteus using anhydrous ammonia and hot water for effective xylan recovery and improved enzymatic saccharification
- Authors
- Cayetano, Roent Dune A.; Kim, Tae Hyun
- Issue Date
- May-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Lignocellulosic biomass; Gaseous ammonia; Pretreatment; Enzymatic digestibility; Fermentable sugar; Hemicellulose hydrolysis
- Citation
- BIORESOURCE TECHNOLOGY, v.255, pp.163 - 170
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 255
- Start Page
- 163
- End Page
- 170
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/6266
- DOI
- 10.1016/j.biortech.2018.01.135
- ISSN
- 0960-8524
- Abstract
- A two-stage method using gaseous ammonia and hot water was proposed to recover xylan and lignin from Miscanthus. In this method, Miscanthus was treated with gaseous ammonia at elevated temperatures (60-150 degrees C) for various reaction times (1-48 h) in the first stage, termed as LMAA (low-moisture anhydrous ammonia) treatment. In the following stage, the LMAA-treated solid was subjected to hot-water treatment in a flow-through column reactor under various reaction conditions (170-220 degrees C, 30-90 min). After two-stage processing, the remaining solid contained mostly glucan (similar to 80% cellulose), which became highly digestible by enzymes. The optimal treatment conditions for sugar recovery using two-stage process were 120 degrees C and 12 h for the 1st stage and 190 degrees C, 90 min, and 5 mL/min for the 2nd stage, which resulted in 84.2% xylan recovery in liquid phase and 95.3% glucan digestibility of the treated solid, using 15 FPU/g-glucan enzyme loading after the two-stage treatment.
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