Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish
- Authors
- Lee, Ji-Hyeok; Ko, Ju-Young; Kim, Eun-A; Hwang, Eun-Kyoung; Park, Chan Sun; Lee, Jung-Suck; Kim, Chul-Young; Lee, Hyi-Seung; Kang, Hee-Kyoung; Cha, Seon-Heui; Jeon, You-Jin
- Issue Date
- Jun-2017
- Publisher
- SPRINGER
- Keywords
- Phaeophyceae, centrifugal partition chromatography; Inflammation; Apo-9 ' -fucoxanthinone; RAW 264.7 cell line; Zebrafish
- Citation
- JOURNAL OF APPLIED PHYCOLOGY, v.29, no.3, pp.1587 - 1596
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF APPLIED PHYCOLOGY
- Volume
- 29
- Number
- 3
- Start Page
- 1587
- End Page
- 1596
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/9570
- DOI
- 10.1007/s10811-016-1012-3
- ISSN
- 0921-8971
- Abstract
- In this study, we isolated and purified secondary metabolites from an edible brown seaweed, Undariopsis peterseniana, and evaluated their anti-inflammatory effects in vitro and in vivo. Apo-9'-fucoxanthinone (Apo-9'), a main anti-inflammatory compound found in the chloroform fraction of U. peterseniana extracts, was first isolated by one-step centrifugal partition chromatography and identified by nuclear magnetic resonance. Apo-9' significantly decreased nitric oxide (NO), prostaglandin E-2, pro-inflammatory proteins (inducible NO synthase [iNOS] and cyclooxygenase [COX-2]), and pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without exhibiting any cytotoxicity. In vivo, Apo-9' protected LPS-stimulated zebrafish embryos against inflammatory stress and suppressed the expression of pro-inflammatory proteins such as COX-2 and iNOS. These findings suggest that U. peterseniana extract and Apo-9' could be used as an anti-inflammatory agent in health care.
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