Antineuroinflammatory and Antiproliferative Activities of Constituents from Tilia amurensis
- Authors
- Kim, Ki Hyun; Moon, Eunjung; Cha, Joon Min; Lee, Seulah; Yu, Jae Sik; Kim, Chung Sub; Kim, Sun Yeou; Choi, Sang Un; Lee, Kang Ro
- Issue Date
- Oct-2015
- Publisher
- PHARMACEUTICAL SOC JAPAN
- Keywords
- Tilia amurensis; Tiliaceae; isoflavonoid glycoside; nitric oxide; antineuroinflammation; antiproliferation
- Citation
- CHEMICAL & PHARMACEUTICAL BULLETIN, v.63, no.10, pp.837 - 842
- Journal Title
- CHEMICAL & PHARMACEUTICAL BULLETIN
- Volume
- 63
- Number
- 10
- Start Page
- 837
- End Page
- 842
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10098
- DOI
- 10.1248/cpb.c15-00393
- ISSN
- 0009-2363
- Abstract
- As part of our ongoing search for bioactive constituents of natural Korean medicinal resources, we found in a preliminary study that the methanol (MeOH) extract from the trunks of Tilia amurensis RUPR. showed an inhibitory effect on nitric oxide (NO) production in an activated murine microglial cell line. A bioassay-guided fractionation and chemical investigation of the MeOH extract resulted in the isolation and identification of a new isoflavonoid glycoside, orobol 4'-O-beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranoside (1) and 16 known compounds (2-17). The structure of the new compound was determined by spectroscopic methods, i.e., one-dimensional (1D) and two-dimensional (2D)-NMR techniques and high resolution (HR)-MS, and chemical methods. The antineuroinflammatory activities of the isolated compounds were determined by measuring NO levels in the medium using murine microglial BV-2 cells. Among them, 12 compounds, including compound 1 (most active with an IC50 value of 23.42 mu M), inhibited NO production in lipopolysaccharide-stimulated BV-2 cells. Moreover, compounds 1-4 showed moderate antiproliferative activities against the SK-MEL-2 cell line, with IC50 values ranging from 12.31 to 19.67 mu M.
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