Characterisation of insulin-producing cells differentiated from tonsil derived mesenchymal stem cells
- Authors
- Kim, So-Yeon; Kim, Ye-Ryung; Park, Woo-Jae; Kim, Han Su; Jung, Sung-Chul; Woo, So-Youn; Jo, Inho; Ryu, Kyung-Ha; Park, Joo-Won
- Issue Date
- Jul-2015
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Insulin; Diabetes; Mesenchymal stem cell; Tonsil; Adipose tissue
- Citation
- DIFFERENTIATION, v.90, no.1-3, pp.27 - 39
- Journal Title
- DIFFERENTIATION
- Volume
- 90
- Number
- 1-3
- Start Page
- 27
- End Page
- 39
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10409
- DOI
- 10.1016/j.diff.2015.08.001
- ISSN
- 0301-4681
- Abstract
- Tonsil-derived (T-) mesenchymal stem cells (MSCs) display mutilineage differentiation potential and self-renewal capacity and have potential as a banking source. Diabetes mellitus is a prevalent disease in modern society, and the transplantation of pancreatic progenitor cells or various stem cell-derived insulin-secreting cells has been suggested as a novel therapy for diabetes. The potential of T-MSCs to trans-differentiate into pancreatic progenitor cells or insulin-secreting cells has not yet been investigated. We examined the potential of human T-MSCs to trans-differentiate into pancreatic islet cells using two different methods based on beta-mercaptoethanol and insulin-transferin-selenium, respectively. First, we compared the efficacy of the two methods for inducing differentiation into insulin-producing cells. We demonstrated that the insulin-transferin-selenium method is more efficient for inducing differentiation into insulin-secreting cells regardless of the source of the MSCs. Second, we compared the differentiation potential of two different MSC types: T-MSCs and adipose-derived MSCs (A-MSCs). T-MSCs had a differentiation capacity similar to that of A-MSCs and were capable of secreting insulin in response to glucose concentration. Islet-like clusters differentiated from T-MSCs had lower synaptotagmin-3, -5, -7, and -8 levels, and consequently lower secreted insulin levels than cells differentiated from A-MSCs. These results imply that T-MSCs can differentiate into functional pancreatic islet-like cells and could provide a novel, alternative cell therapy for diabetes mellitus. (C) 2015 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
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