Anti-inflammatory and antitumor phenylpropanoid sucrosides from the seeds of Raphanus sativus
- Authors
- Kim, Ki Hyun; Kim, Chung Sub; Park, Yong Joo; Moon, Eunjung; Choi, Sang Un; Lee, Jei Hyun; Kim, Sun Yeou; Lee, Kang Ro
- Issue Date
- 1-Jan-2015
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Raphanus sativus; Brassicaceae; Sucrose derivatives; Anti-inflammation; Cytotoxicity; Antioxidant activity
- Citation
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.25, no.1, pp.96 - 99
- Journal Title
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- Volume
- 25
- Number
- 1
- Start Page
- 96
- End Page
- 99
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10870
- DOI
- 10.1016/j.bmcl.2014.11.001
- ISSN
- 0960-894X
- Abstract
- A bioassay-guided fractionation and chemical investigation of the MeOH extract of Raphanus sativus seeds resulted in the isolation and identification of eight phenylpropanoid sucrosides (1-8) including two new compounds, named raphasativuside A and B (1-2) from the most active CHCl3-soluble fraction. The structures of these new compounds were elucidated through spectral analysis, including extensive 2D-NMR data, and chemical reaction experiments. We evaluated the anti-inflammatory effects of 1-8 in lipopolysaccharide (LPS)-stimulated murine microglia BV2 cells. Compounds 2 and 5 exhibited significant inhibitory effect on nitric oxide production in LPS-activated BV-2 cells with IC50 values of 21.63 and 26.96 mu M, respectively. All isolates were also evaluated for their antiproliferative activities against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, and HCT-15). Compounds 1-7 showed consistent cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines with IC50 values of 6.71-27.92 mu M. Additionally, the free-radical scavenging activity of 1-8 was assessed using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay where compounds 1, 3, and 4 scavenged DPPH radical strongly with IC50 values of 23.05, 27.10, and 29.63 mu g/mL, respectively. (C) 2014 Elsevier Ltd. All rights reserved.
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