One-step nucleic acid extraction and amplification on an integrated microdevice for early detection of cochlodinium polykrikoides
- Authors
- Nguyen, T.P.O.; Jung, S.W.; Trinh, K.T.L.; Tran, B.M.; Pham, Q.N.; Kim, J.-H.; Lee, N.Y.
- Issue Date
- Oct-2015
- Publisher
- Chemical and Biological Microsystems Society
- Keywords
- Amine-functionalized; Cochlodinium polykrikoides; Monolithic microdevice
- Citation
- MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp.1274 - 1276
- Journal Title
- MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- Start Page
- 1274
- End Page
- 1276
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10996
- ISSN
- 0000-0000
- Abstract
- Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell - Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea - was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection. © 15CBMS-0001.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 산업·환경대학원 > 산업환경공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/10996)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.