Characterization of Xylanase from Bacillus agaradhaerens DK-2386 Isolated from Korean Soil
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, J.-H. | - |
dc.contributor.author | Park, Y.-S. | - |
dc.contributor.author | Lee, H. | - |
dc.contributor.author | Bai, D.-H. | - |
dc.date.available | 2020-02-28T11:41:24Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1598-642X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11060 | - |
dc.description.abstract | The optimum conditions for the production of xylanase from Bacillus agaradhaerens DK-2386 have been previously investigated. In this study xylanase was purified by ammonium sulfate precipitation and CM-sepharose ion exchange chromatography. The molecular mass of the xylanase as determined by SDS-PAGE was 23 kDa in a form of monomeric enzyme. The optimum pH and temperature for xylanase activity was 6.0 and 60°C, respectively. Xylanase activity was increased by the addition of EDTA and then stabilized at 40°C for 24 h. The maximum xylanase activity was obtained when Birchwood xylan was used as a substrate and the Vmax and Km were 49,724 μmol/min and 6.08 mg/ml, respectively. © 2015, The Korean Society for Microbiology and Biotechnology. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | Korean Society for Microbiolog and Biotechnology | - |
dc.relation.isPartOf | Korean Journal of Microbiology and Biotechnology | - |
dc.title | Characterization of Xylanase from Bacillus agaradhaerens DK-2386 Isolated from Korean Soil | - |
dc.title.alternative | 토양으로부터 분리한 Bacillus agaradhaerens DK-2386 균주가 생산하는 Xylanase의 특성 | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.doi | 10.4014/mbl.1511.11002 | - |
dc.identifier.bibliographicCitation | Korean Journal of Microbiology and Biotechnology, v.43, no.4, pp.330 - 335 | - |
dc.identifier.kciid | ART002059006 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-84956615165 | - |
dc.citation.endPage | 335 | - |
dc.citation.startPage | 330 | - |
dc.citation.title | Korean Journal of Microbiology and Biotechnology | - |
dc.citation.volume | 43 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | Park, Y.-S. | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Activity | - |
dc.subject.keywordAuthor | Bacillus | - |
dc.subject.keywordAuthor | Purification | - |
dc.subject.keywordAuthor | Xylanase | - |
dc.subject.keywordPlus | edetic acid | - |
dc.subject.keywordPlus | sepharose | - |
dc.subject.keywordPlus | xylan endo 1,3 beta xylosidase | - |
dc.subject.keywordPlus | ammonium sulfate | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | Bacillus | - |
dc.subject.keywordPlus | Bacillus agaradhaerens | - |
dc.subject.keywordPlus | ion exchange chromatography | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | polyacrylamide gel electrophoresis | - |
dc.subject.keywordPlus | precipitation | - |
dc.subject.keywordPlus | soil | - |
dc.subject.keywordPlus | bacterium isolation | - |
dc.subject.keywordPlus | enzyme activity | - |
dc.subject.keywordPlus | enzyme analysis | - |
dc.subject.keywordPlus | molecular weight | - |
dc.subject.keywordPlus | pH | - |
dc.subject.keywordPlus | soil | - |
dc.subject.keywordPlus | South Korea | - |
dc.subject.keywordPlus | temperature | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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