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Characterization of Xylanase from Bacillus agaradhaerens DK-2386 Isolated from Korean Soil토양으로부터 분리한 Bacillus agaradhaerens DK-2386 균주가 생산하는 Xylanase의 특성
- Other Titles
- 토양으로부터 분리한 Bacillus agaradhaerens DK-2386 균주가 생산하는 Xylanase의 특성
- Authors
- Choi, J.-H.; Park, Y.-S.; Lee, H.; Bai, D.-H.
- Issue Date
- Dec-2015
- Publisher
- Korean Society for Microbiolog and Biotechnology
- Keywords
- Activity; Bacillus; Purification; Xylanase
- Citation
- Korean Journal of Microbiology and Biotechnology, v.43, no.4, pp.330 - 335
- Journal Title
- Korean Journal of Microbiology and Biotechnology
- Volume
- 43
- Number
- 4
- Start Page
- 330
- End Page
- 335
- ISSN
- 1598-642X
- Abstract
- The optimum conditions for the production of xylanase from Bacillus agaradhaerens DK-2386 have been previously investigated. In this study xylanase was purified by ammonium sulfate precipitation and CM-sepharose ion exchange chromatography. The molecular mass of the xylanase as determined by SDS-PAGE was 23 kDa in a form of monomeric enzyme. The optimum pH and temperature for xylanase activity was 6.0 and 60°C, respectively. Xylanase activity was increased by the addition of EDTA and then stabilized at 40°C for 24 h. The maximum xylanase activity was obtained when Birchwood xylan was used as a substrate and the Vmax and Km were 49,724 μmol/min and 6.08 mg/ml, respectively. © 2015, The Korean Society for Microbiology and Biotechnology.
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Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
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Related Researcher
- Park, Young Seo
- BioNano Technology (Department of Food Science & Biotechnology)