Nanoclusters prepared from ruthenium(II) and quercetin for fluorometric detection of cobalt(II), and a method for screening their anticancer drug activity
- Authors
- Lakshmi, Buddolla Anantha; Bae, Jin-Young; An, Jeong Ho; Kim, Sanghyo
- Issue Date
- Aug-2019
- Publisher
- SPRINGER WIEN
- Keywords
- Atomic force microscopy; Bioimaging; Cytotoxicity; Flavonoids; Fluorescent probe; Intracellular fluorosensing; Live-dead staining
- Citation
- MICROCHIMICA ACTA, v.186, no.8
- Journal Title
- MICROCHIMICA ACTA
- Volume
- 186
- Number
- 8
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1160
- DOI
- 10.1007/s00604-019-3657-5
- ISSN
- 0026-3672
- Abstract
- Ruthenium-quercetin conjugated nanoclusters (Ru-QC NCs) were synthesized via a one-pot reflux reaction. As inhalation of heavy metal ions like cobalt can lead to lung cancer, a fluorescent probe was designed for the determination of Co(II) both in aqueous solutions and living cells. The probe consists of hybrid nanoclusters with an average size of 2 nm that were prepared from ruthenium(II) ions and the flavonoid quercetin. These are termed as Ru-QC NCs. They display strong orange-colored emission with a peak at 558 nm under 465-nm excitation. The Ru-QC NCs are cell viable and enable imaging of cells and intracellular fluorometric detection of Co(II). The anticancer properties of Ru-QC NCs were screened by using non-small cell lung cancer (A549) and human dermal fibroblast (HDFa) cell lines. The Ru-QC NCs exert considerable cytotoxicity in A549 cells (at levels of 20-50 mu g.mL(-1)), whereas no significant cytotoxicity was observed in case of HDFa cells. The anticancer properties of Ru-QC NCs were screened via MTT assay, live-dead staining, and ROS assay, respectively. Morphological changes of cancer cells were observed using atomic force microscopy. The fluorescent probe can detect Co(II) with a detection limit of 9.28 nM and with a linear response in the 0.03-100 mu M concentration range.
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