Different inhibition properties of catechins on the individual subunits of mucosal alpha-glucosidases as measured by partially-purified rat intestinal extract
- Authors
- Lim, Jongbin; Kim, Do Kyoung; Shin, Hansol; Hamaker, Bruce R.; Lee, Byung-Hoo
- Issue Date
- 1-Jul-2019
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- FOOD & FUNCTION, v.10, no.7, pp.4407 - 4413
- Journal Title
- FOOD & FUNCTION
- Volume
- 10
- Number
- 7
- Start Page
- 4407
- End Page
- 4413
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1255
- DOI
- 10.1039/c9fo00990f
- ISSN
- 2042-6496
- Abstract
- Mucosal alpha-glucosidases from rat intestinal powder were employed, with a step to remove alpha-amylase, to measure the possibility of different inhibition of catechins, particularly those found in tea, on the four alpha-glucosidase enzymes. Inhibition of catechins was investigated for the slowing of digestion of glycemic carbohydrates, thus regulating glucose release and absorption. The alpha-glucosidases were fractionated using size-exclusion chromatography. The partially purified fractions showed higher alpha-glucosidase activity without any alpha-amylase activity. Catechins had selective inhibition properties on the alpha-glucosidases. In particular, (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) showed comparably high inhibitory effect on all four individual alpha-glucosidases, while (-)-epicatechin (EC), and (+)-catechin (C) indicated a more discriminating effect with relatively higher inhibitory effects on sucrase-isomaltase. The findings suggest that catechins differently inhibit the individual subunits of the alpha-glucosidases, and that they could modulate postprandial blood glucose levels through slowing digestion rate of starch and other glycemic carbohydrates, including sucrose.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 식품생물공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1255)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.