黃連 추출물의 항염효능에 관한 연구Anti-inflammatory Effect of Coptidis Rhizoma Extract
- Other Titles
- Anti-inflammatory Effect of Coptidis Rhizoma Extract
- Authors
- 이전우; 한효상; 이영종
- Issue Date
- 2014
- Publisher
- 대한본초학회
- Keywords
- Coptidis Rhizoma; macrophage cells; cytokine; nitric oxide; JNK
- Citation
- 대한본초학회지, v.29, no.5, pp.83 - 90
- Journal Title
- 대한본초학회지
- Volume
- 29
- Number
- 5
- Start Page
- 83
- End Page
- 90
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13442
- ISSN
- 1229-1765
- Abstract
- Objectives : This research has been done to investigate the anti-inflammatory effect of Coptidis Rhizoma extracts.
Method : Coptidis Rhizoma was extracted by 100℃ water. The extract (CC : Extract of Coptis chinensis rhizome) was used to examine its effects on the cell viability of mouse macrophage Raw 264.7 cell line. Also the production of nitric oxide (NO), the c-jun N-terminalkinase (JNK) activation and the production of cytokines such as (IL)-5 were evaluated in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. After the CC and LPS were applied to Raw 264.7 cells which were cultured for 24 hours, the MTT assay was performed.
Result : The CC extracts didn't affect the viability of macrophage cells. However, the extracts inhibited the NO production and the JNK activation significantly in LPS-stimulated macrophage cells treated with 100 and 200 ㎍/mL concentrations. The CC extract, also, impeded the production of inflammation-related factors and cytokines such as KC, VEGF, MCP-1, GM-CSF, IL-1α, IL-5, IL-6, and IL-12p40 in LPS-stimulated macrophage cells at the concentration higher than 25 ㎍/mL. The production of basic-FGF concentration of 50 and 100 ㎍/mL, the production of IP-10 at 100 ㎍/mL, and the production of IFN-γ at 25 ㎍/mL, respectively.
Conclusion : The CC prepared using 100℃ water showed the significant anti-inflammatory effect such as the inhibition not only on the production of NO, KC, VEGF, MCP-1, GM-CSF, IL-1α, IL-5, IL-6, and IL-12p40 in LPS-stimulated macrophage cells at or higher than the concentration of 25 ㎍/mL, but also on the JNK activation at 100 and 200 ㎍/mL.
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