황련해독탕(黃連解毒湯)이 외열 스트레스(火邪)에 의한 동물모델에서 c-fos 발현에 미치는 영향 연구

Abstract

Objectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherbprescription. We evaluated the protective effect of HH against heat stress exposure in mice. Methods: Six weeks old ICR mice were used for this study. After 43℃ heat stressfor 15 minutes, we evaluated the changes of motor activity and c-fos expressionlevel to determine the proper heat stress and evaluation time. The subjects weredivided into 4 groups (1. control group, 2. heat stress group with normal saline,3. heat stress group with administration of 100 mg/kg, and 4. heat stress group withadministration of 1000 mg/kg). After oral administration of HH once a day for 3 days,2, 3, 4 groups were exposed to 43℃ heat stress for 15 minutes. Then, we evaluatedthe motor activity for 120 minutes and analyzed the c-fos expression using westernblot. We investigated the effect of HH and its ingredients on c-fos expressions afterheat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma,Phellodendri Cortex, and Gardeniae Fructus for 3 days. After one hour of last treatment,the mice were exposed to heat stress at 43℃ for 15 min. After two hours, the hypothalamuswas dissociated and lysed to measure c-fos expression. Results: After oral administration of HH for 3 days, motor activity was recoveredinsignificantly. 100 mg/kg HH treatment reduced the c-fos expression after heat stressbut insignificantly. Among the ingredients of HH, Coptidis Rhizoma and ScutellariaeRadix treatment groups significantly reduced the c-fos after stress. Conclusions: These results show that Hwangryunhaedok-tang may be effectiveto reduce the heat stress response.