황련해독탕(黃連解毒湯)이 외열 스트레스(火邪)에 의한 동물모델에서 c-fos 발현에 미치는 영향 연구The Effect of Hwangryunhaedok-tang on the c-fos Level in Mice Exposed to Heat Stress
- Other Titles
- The Effect of Hwangryunhaedok-tang on the c-fos Level in Mice Exposed to Heat Stress
- Authors
- 황덕상; 김효근; 오명숙; 김태미; 이태희
- Issue Date
- 2014
- Publisher
- 대한한방부인과학회
- Keywords
- Heat Stress; Hwangryunhaedok-tang; Motor Activity; C-fos; Coptidis Rhizoma; Scutellariae Radix
- Citation
- 대한한방부인과학회지, v.27, no.3, pp.1 - 11
- Journal Title
- 대한한방부인과학회지
- Volume
- 27
- Number
- 3
- Start Page
- 1
- End Page
- 11
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13465
- DOI
- 10.15204/jkobgy.2014.27.3.001
- ISSN
- 1229-4292
- Abstract
- Objectives: Hwangryunhaedok-tang (HH) is a representative heat clearing multiherbprescription. We evaluated the protective effect of HH against heat stress exposure in mice.
Methods: Six weeks old ICR mice were used for this study. After 43℃ heat stressfor 15 minutes, we evaluated the changes of motor activity and c-fos expressionlevel to determine the proper heat stress and evaluation time. The subjects weredivided into 4 groups (1. control group, 2. heat stress group with normal saline,3. heat stress group with administration of 100 mg/kg, and 4. heat stress group withadministration of 1000 mg/kg). After oral administration of HH once a day for 3 days,2, 3, 4 groups were exposed to 43℃ heat stress for 15 minutes. Then, we evaluatedthe motor activity for 120 minutes and analyzed the c-fos expression using westernblot. We investigated the effect of HH and its ingredients on c-fos expressions afterheat stress. The mice were administrated HH, Scutellariae Radix, Coptidis Rhizoma,Phellodendri Cortex, and Gardeniae Fructus for 3 days. After one hour of last treatment,the mice were exposed to heat stress at 43℃ for 15 min. After two hours, the hypothalamuswas dissociated and lysed to measure c-fos expression.
Results: After oral administration of HH for 3 days, motor activity was recoveredinsignificantly. 100 mg/kg HH treatment reduced the c-fos expression after heat stressbut insignificantly. Among the ingredients of HH, Coptidis Rhizoma and ScutellariaeRadix treatment groups significantly reduced the c-fos after stress.
Conclusions: These results show that Hwangryunhaedok-tang may be effectiveto reduce the heat stress response.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 한의과대학 > 한의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13465)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.