Fully integrated and slidable paper-embedded plastic microdevice for point-of-care testing of multiple foodborne pathogens
- Authors
- Kieu The Loan Trinh; Thi Ngoc Diep Trinh; Lee, Nae Yoon
- Issue Date
- Jun-2019
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Integrated microdevice; Foodborne pathogens; DNA extraction; Loop-mediated isothermal amplification (LAMP); Colorimetric detection
- Citation
- BIOSENSORS & BIOELECTRONICS, v.135, pp.120 - 128
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 135
- Start Page
- 120
- End Page
- 128
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1357
- DOI
- 10.1016/j.bios.2019.04.011
- ISSN
- 0956-5663
- Abstract
- This study presents a slidable paper-embedded plastic microdevice fully integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities. The developed microdevice consists of three layers that allow a sliding movement to mix the sample and reagents for DNA purification, amplification, and detection in a sequential manner. An FTA card was employed in the main chamber for DNA extraction and purification from intact bacterial cells. Subsequently, LAMP reagents and fuchsin-stored chambers were pulled toward the main chambers for DNA amplifications at 65 degrees C. After 30 min, the detection reagents-stored chambers were then moved to main chambers for result analysis. For the detection of LAMP amplicons, a novel colorimetric fuchsin-based method was employed. The wide applicability of the integrated microdevice was demonstrated by successfully screening three major foodborne pathogens, namely Salmonella spp., Staphylococcus aureus, and Escherichia coli O157:H7 in food, enabling highly sensitive detection of 3.0 x 10(1) CFU/sample of Gram-negative bacteria (Salmonella spp. and Escherichia coli O157:H7) and 3.0 x 10(2) CFU/sample of Gram-positive bacteria (Staphylococcus aureus) within 75 min. The portable and integrated microdevice presented in this study holds significant promise for point-of-care applications to accurately and rapidly diagnose and control diseases.
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Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 산업·환경대학원 > 산업환경공학과 > 1. Journal Articles
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