위령선 추출물이 Human Neuroblastoma 세포주에서 CoCl2에 의해 유도된 세포사멸에 미치는 보호효과Protective Effect of Clematidis Radix Extract on CoCl2-induced Apoptosis in Human Neuroblastoma Cells
- Other Titles
- Protective Effect of Clematidis Radix Extract on CoCl2-induced Apoptosis in Human Neuroblastoma Cells
- Authors
- 박정우; 임형호
- Issue Date
- 2014
- Publisher
- 한방재활의학과학회
- Keywords
- Clematidis radix; CoCl2; Hypoxia; Apoptosis; SH-SY5Y human neuro-blastoma cells
- Citation
- 한방재활의학과학회지, v.24, no.2, pp.41 - 50
- Journal Title
- 한방재활의학과학회지
- Volume
- 24
- Number
- 2
- Start Page
- 41
- End Page
- 50
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13742
- ISSN
- 1229-1854
- Abstract
- Objectives The purpose of this study was to evaluate the effects of Clematidis radix extract on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells.
Methods In order to investigate the protective effect of Clematidis radix on CoCl2-induced cytotoxicity in neuronal cells, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI(4,6-diamidino-2-phenylindoleI) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay and western blotting were performed on SH-SY5Y human neuroblastoma cells.
Results Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treated with Clematidis radix prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, in contrast, Clematidis radix treatment decreased CoCl2-induced HIF-1α expression. Pre-treatment with the extract of Clematidis radix suppressed Bax, cytochrome c, and caspase-3 expressions, and also increased Bcl-2 expression in SH-SY5Y human neuroblastoma cells.
Conclusions These results suggest that Clematidis radix may exert a protective effect on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells.
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