Herbal Prescription, DSGOST, Prevents Cold-Induced RhoA Activation and Endothelin-1 Production in Endothelial Cells
- Authors
- Cho, Sung-Gook; Go, Ho Yeon; Park, Jeong-Su; Jung, Ki-Yong; Sun, Seung-Ho; Choi, You-Kyung; Song, Yun-Kyung; Park, Jong-Hyeong; Jun, Chan-Yong; Ko, Seong Gyu
- Issue Date
- 2014
- Publisher
- HINDAWI LTD
- Citation
- EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
- Journal Title
- EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14002
- DOI
- 10.1155/2014/549307
- ISSN
- 1741-427X
- Abstract
- Herbal prescription, Danggui-Sayuk-Ga-Osuyu-Saenggang-tang (DSGOST), has long been used to treat Raynaud's phenomenon (RP) in traditional Chinese medicine (TCM). However, a biological mechanism by which DSGOST ameliorates RP is yet deciphered. In this study, we demonstrate that DSGOST inhibits cold-induced activation of RhoA, in both vascular smooth-muscle cells (VSMC) and endothelial cells (EC), and blocks endothelin-1-mediated paracrine path for cold response on vessels. While cold induced RhoA activity in both cell types, DSGOST pretreatment prevented cold-induced RhoA activation. DSGOST inhibition of cold-induced RhoA activation further blocked. alpha 2c-adrenoreceptor translocation to the plasma membrane in VSMC. In addition, DSGOST inhibited endothelin-1-mediated RhoA activation and. alpha 2c-adrenoreceptor translocation in VSMC. Meanwhile, DSGOST inhibited cold-induced or RhoA-dependent phosphorylation of FAK, SRC, and ERK. Consistently, DSGOST inhibited cold-induced endothelin-1 expression in EC. Therefore, DSGOST prevents cold-induced RhoA in EC and blocks endothelin-1-mediated paracrine path between EC and VSMC. In conclusion, our data suggest that DSGOST is beneficial for treating RP-like syndrome.
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Collections - 한의과대학 > 한의학과 > 1. Journal Articles
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