Influence of DNA Concentration on the Interfacial Electrode Impedance
- Authors
- Cho, Sungbo; Oh, Younshin; Ahn, Sung-Min
- Issue Date
- Nov-2013
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- DNA Quantification; Equivalent Circuit; Fitting Analysis; Impedance Spectroscopy
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.13, no.11, pp.7291 - 7294
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 13
- Number
- 11
- Start Page
- 7291
- End Page
- 7294
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14179
- DOI
- 10.1166/jnn.2013.8085
- ISSN
- 1533-4880
- Abstract
- Sensitive and accurate DNA quantification is an essential step in most DNA analyses, especially in analysis of a small amount of DNA. Instead of typical DNA quantification methods (i.e., UV spectrometry, fluorometry, and quantitative polymerase chain reaction), electrical impedance spectroscopy has recently been investigated for label-free and efficient quantification of DNA. In this study, quantification of DNA in solution was investigated using electrical impedance spectroscopy without pre-processing, including the modification of the electrode surface and immobilization of DNA onto the electrode. The results showed that the measured impedance spectra were in agreement with a designed equivalent circuit model, and that the extrapolated parameters of interfacial electrode impedance were dependent on DNA concentration. The impedance measured at 1 and 10 kHz showed good correlation with DNA concentration (R-2 > 0.95), with a dynamic range of detection from 0.01 pg/mu l to 100 ng/mu l. Based on the results, it is expected to utilize impedance spectroscopy for the real-time and label-free characterization of DNA concentration with high sensitivity and low operation cost.
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