Pump less steady-flow microfluidic chip for cell culture
- Authors
- Marimuthu, Mohana; Kim, Sanghyo
- Issue Date
- 15-Jun-2013
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Microfluidic; Bioreactor; Perfusion; Flow rate; Dermal fibroblasts
- Citation
- ANALYTICAL BIOCHEMISTRY, v.437, no.2, pp.161 - 163
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 437
- Number
- 2
- Start Page
- 161
- End Page
- 163
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14472
- DOI
- 10.1016/j.ab.2013.02.007
- ISSN
- 0003-2697
- Abstract
- The current research engineered a pumpless energy-efficient microfluidic perfusion cell culture chip that works by modifying the basic gravity-driven siphon flow using an intravenous (IV) infusion set as a conventional, inexpensive, and sterile tool. The IV set was modified to control the constant hydrostatic head difference, thereby maintaining the steady flow rate medium perfusion. The micro-bioreactor chip demonstrated flexibility in controlling a wide range of flow rates from 0.1 to 10 ml/min, among which 1- and 5-ml/min flow rates were examined as suitable shear flows for long-term dermal fibroblast cell culture, paving the way for artificial skin development. (C) 2013 Elsevier Inc. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.