Capacitive immunoaffinity biosensor based on vertically paired ring-electrodes
- Authors
- Lee, Ga-Yeon; Choi, Yong-Hwan; Chung, Ha-Wook; Ko, Hyuk; Cho, Sungbo; Pyun, Jae-Chul
- Issue Date
- 15-Feb-2013
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Capacitive; Immunoassay; Non-labeled detection; Parylene-C; Impedance
- Citation
- BIOSENSORS & BIOELECTRONICS, v.40, no.1, pp.227 - 232
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 40
- Number
- 1
- Start Page
- 227
- End Page
- 232
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14747
- DOI
- 10.1016/j.bios.2012.07.028
- ISSN
- 0956-5663
- Abstract
- A capacitive biosensor was developed by using a vertically paired ring-electrode for the non-labeled immunoassay. The vertically paired ring-electrode was prepared by sequential deposition and etching processes. Two electrodes were layered on glass substrate by sputtering of gold layers with thicknesses of 50 nm and 100 nm, and a parylene-C film with a thickness of 550 nm was positioned between the electrode layers as a dielectric material by thermal deposition. The top layer was made by spin coating of SU-8. And then, the ring-electrodes were exposed at the wall of the layered structure by sequential etching processes. The fabricated electrodes were characterized by cyclic voltammetry of a well-known redox couple of 3,3',5,5'-tetramethylbenzidine. The non-labeled detection of antigen-antibody interaction was demonstrated by using anti-horseradish peroxidase (HRP) antibodies and C-reactive protein (CRP) as model analytes. When the model analytes were bound to the vertically paired ring-electrode, the impedance change was measured during the immunoassay steps, and the measured impedance was analyzed by using a model circuit of the ring-electrode, and the capacitance was estimated to be dependent on the adsorption of analytes between the ring-electrodes. (C) 2012 Elsevier B.V. All rights reserved.
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