One-step solid phase-based on-chip sample preparation and integration with flow-through polymerase chain reaction
- Authors
- Trinh, K.T.L.; Tran, H.H.; Zhang, Y.; Wu, J.; Lee, N.Y.
- Issue Date
- Oct-2013
- Publisher
- Chemical and Biological Microsystems Society
- Keywords
- Chelex resin; Flow-through polymerase chain reaction; Integrated plastic microdevice; Sample preparation
- Citation
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, v.1, pp.341 - 343
- Journal Title
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- Volume
- 1
- Start Page
- 341
- End Page
- 343
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14880
- ISSN
- 0000-0000
- Abstract
- Sample preparation is one of the most labor-intensive and time-consuming processes yet indispensable for realizing a truly integrated micro total analyses systems (μTAS). In this study, we introduce Chelex resin as a solid support for one- step sample preparation. A plastic microdevice was fabricated using poly(methylmethacrylate) (PMMA), and Chelex resin was physically captured inside a microchannel. Using the fabricated microdevice, DNA was successfully extracted and purified from Escherichia coli and from human hairs. Furthermore, the sample preparation system was functionally integrated with flow-through polymerase chain reaction unit, and E. coli was successfully amplified in a seamless flow using the integrated microdevice. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 산업·환경대학원 > 산업환경공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14880)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.