One-step solid phase-based on-chip sample preparation and integration with flow-through polymerase chain reaction

Abstract

Sample preparation is one of the most labor-intensive and time-consuming processes yet indispensable for realizing a truly integrated micro total analyses systems (μTAS). In this study, we introduce Chelex resin as a solid support for one- step sample preparation. A plastic microdevice was fabricated using poly(methylmethacrylate) (PMMA), and Chelex resin was physically captured inside a microchannel. Using the fabricated microdevice, DNA was successfully extracted and purified from Escherichia coli and from human hairs. Furthermore, the sample preparation system was functionally integrated with flow-through polymerase chain reaction unit, and E. coli was successfully amplified in a seamless flow using the integrated microdevice. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved.