LPS로 유발된 마우스 대식세포의 케모카인류 염증인자 생성에 미치는 백삼이중탕 및 홍삼이중탕의 영향비교

Abstract

The purpose of this study is to investigate effects of White Ginseng-Ejung-tang (EG) and Red Ginseng-Ejung-tang (ER) water extract on production of various cytokines such as interleukin (IL)-21, IL-25, IL-28β, erythropoietin (EPO), Exodus-2, monocyte chemotactic protein (MCP)-5, macrophage inflammatory protein (MIP)-3α, MIP-3β, Fractalkine, and TARC in RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS). Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. ER significantly decreased levels of IL-21, IL-25, IL-28β, EPO, Exodus-2, MCP-5, MIP-3α, MIP-3β, TARC, and fractalkine for 24 h incubation at the oncentrations of 25 and 100 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). But EG did not show any significant effect. These results suggest that ER has anti-inflammtory property related with its inhibition on the production of IL-21, IL-25, IL-28β, and chemokines such as EPO, MCP-5, MIP-3α, MIP-3β, Fractalkine, Exodus-2, and TARC in LPS-induced macrophages.