女貞實이 LPS로 유발된 RAW 264.7 cell의 염증에 미치는 영향Anti-Inflammatory Effect of Ligustri Lucidi Fructus Water Extract in RAW 264.7 Cells Induced by LPS
- Other Titles
- Anti-Inflammatory Effect of Ligustri Lucidi Fructus Water Extract in RAW 264.7 Cells Induced by LPS
- Authors
- 이용현; 임은미
- Issue Date
- 2013
- Publisher
- 대한한방부인과학회
- Keywords
- Ligustri Lucidi Fructus; NO; Cytokine; Ca; Anti-inflammation
- Citation
- 대한한방부인과학회지, v.26, no.4, pp.66 - 81
- Journal Title
- 대한한방부인과학회지
- Volume
- 26
- Number
- 4
- Start Page
- 66
- End Page
- 81
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15184
- ISSN
- 1229-4292
- Abstract
- Purpose: This study was carried out to investigate the anti-inflammatory effectsof Ligustri Lucidi Fructus water extract (LF) in the lipopolysaccharide (LPS)-inducedmouse macrophages RAW 264.7 cell.
Methods: Ligustri Lucidi Fructus was extracted with distilled water (2,000 ml)for 2 hours. In order to evaluate cytotoxicity of LF, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed. To investigateanti-inflammatory effects of LF, the concentration of nitric oxide (NO) was measuredwith NO assay, cytokine was measured by Bio-Plex cytokine assay, and intracellularcalcium (Ca) was measured with Fluo-4 Ca assay in RAW 264.7 cell. And when p-valueis below 0.05, it is judged to have the significant difference statistically (P<0.05).
Results:1. LF showed no cytotoxicity.
2. LF inhibited significantly the production of NO at the concentration of 25,50 and 100 μg/ml.
3. LF inhibited significantly the production of interleukin (IL)-4, macrophageinflammatory protein (MIP)-1α, granulocyte colony stimulating factor (G-CSF)at the concentration of 25, 50, 100 and 200 μg/ml.
4. LF inhibited significantly the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF), vascular endothelial growth factor (VEGF) at the concentrationof 50, 100 and 200 μg/ml, the interferon (IFN)-γ at 25, 50 and 100 μg/ml respectively.
5. LF inhibited significantly the production of IL-1β at the concentration of 50and 200 μg/ml, the IL-5 at 25 and 100 μg/ml, the IL-12p70, MIP-1β at 50 and100 μg/ml, the regulated on activation, normal T cell expressed and secrete d(RANTES) at 100 and 200 μg/ml respectively.
6. LF inhibited significantly the production of IL-10, interferon gamma-inducedprotein (IP)-10 at the concentration of 200 μg/ml.
7. LF inhibited significantly the production of intracellular Ca at the concentrationof 25, 50, 100 and 200 μg/ml.
Conclusions: These results suggest that LF has anti-inflammatory effect andimmuno-modulating activity.
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