Effects of Ginsenoside-Rg1 on Post-thawed Miniature Pig Sperm Motility, Mitochondria Activity, and Membrane Integrity
- Authors
- 황유진; 김대영
- Issue Date
- 2013
- Publisher
- 사단법인 한국동물생명공학회
- Keywords
- ginsenoside-Rg1; pig sperm; CASA; mitochondria activity; membrane integrity
- Citation
- 한국동물생명공학회지, v.28, no.1, pp.63 - 71
- Journal Title
- 한국동물생명공학회지
- Volume
- 28
- Number
- 1
- Start Page
- 63
- End Page
- 71
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15649
- ISSN
- 2671-4639
- Abstract
- In this study, we used flow a cytometric assay to evaluate plasma membrane integrity and mitochondrial activity in post-thawed sperm that was supplemented with ginsenoside-Rg1. Varying concentrations of ginsenoside-Rg1 (0, 25, 50 and 100 μM/ml) were used in the extender during cryopreservation to protect the DNA of thawed sperm, thereby increasing the viability and motility rate as evaluated using a computer-assisted sperm analysis (CASA) method. The results derived from CASA were used to compare the fresh, control, and ginsenoside-Rg1 groups. Sperm motility and the number of progressively motile sperm were significantly (p<0.05) higher in the 50 μM/ml ginsenoside-Rg1 group (61.0±4.65%) than in the control (46.6±7.02%), 25 μM/ml (46.2±4.76%), and 100 μM/ml ginsenoside-Rg1 (52.0± 1.90%) groups. However, the velocity distribution of post-thawed sperm did not differ significantly. Membrane integrity and MMP staining as revealed using flow cytometry were significantly (p<0.05) higher (91.6±0.82%) in the 50 μM/ml ginsenoside-Rg1 group than in the other groups. Here, we report that ginsenoside-Rg1 affects the motility and viability of boar spermatozoa. Moreover, ginsenoside- Rg1 can be used as a protective additive for the suppression of intracellular mitochondrial oxidative stress caused by cryopreservation
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Collections - 바이오나노대학 > 생명과학과 > 1. Journal Articles
- 보건과학대학 > 의용생체공학과 > 1. Journal Articles
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