Inhibitory Effect of Snake Venom Toxin on Colorectal Cancer HCT116 Cells Growth through Induction of Intrinsic or Extrinsic Apoptosis
- Authors
- 김경태; 송호섭
- Issue Date
- 2013
- Publisher
- 대한침구의학회
- Keywords
- SVT; TRAIL; DR4; DR5; ROS
- Citation
- Journal of Acupuncture Research, v.30, no.1, pp.43 - 55
- Journal Title
- Journal of Acupuncture Research
- Volume
- 30
- Number
- 1
- Start Page
- 43
- End Page
- 55
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/15849
- ISSN
- 2586-288X
- Abstract
- I investigated whether snake venom toxin(SVT) from Vipera lebetina turanica enhances the apoptosis ability of tumor necrosis factor(TNF)-related apoptosis-inducing ligand(TRAIL) in cancer cells. TRAIL inhibited HCT116 cell growth in a dose-dependent manner. Consistent with cell growth inhibition, the expression of TRAIL receptors; DR4 and DR5 was significantly increased as well as apoptosis related proteins such as cleaved caspase-3, 8, 9 and Bax. However, the expression of survival proteins(eg, cFLIP, survivin, XIAP and Bcl2) was suppressed by the combination treatment of SVT and TRAIL. Pretreatment with the reactive oxygen species(ROS) scavenger N-acetylcysteine reduced the SVT and TRAIL-induced upregulation of DR4 and DR5 expression and expression of the apoptosis related protein such as caspase-3 and-9 as well as cell growth inhibitory effects. The collective results suggest that SVT facilitates TRAIL-induced apoptosis in human colorectal cancer HCT116 cells through up-regulation of the TRAIL receptors; DR4 and DR5 via ROS pathway signals.
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Collections - 한의과대학 > 한의학과 > 1. Journal Articles
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