Electrochemical performance of gold nanoparticle-cytochrome c hybrid interface for H2O2 detection
- Authors
- Yagati, Ajay Kumar; Lee, Taek; Min, Junhong; Choi, Jeong-Woo
- Issue Date
- 1-Apr-2012
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Cytochrome c; Gold nanoparticle; Atomic force microscopy; Cyclic voltammetry; Biosensor
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v.92, pp.161 - 167
- Journal Title
- COLLOIDS AND SURFACES B-BIOINTERFACES
- Volume
- 92
- Start Page
- 161
- End Page
- 167
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16448
- DOI
- 10.1016/j.colsurfb.2011.11.035
- ISSN
- 0927-7765
- Abstract
- Here, we describe the formation of a hybrid biointerface consisting of gold nanoparticle (AuNP) and cytochrome c (cyt c) on indium tin oxide (ITO) electrodes using a two-step immobilization procedure. The Au nanoparticles were attached to the ITO electrodes by 3-mercaptopropyl trimethoxysilane (3-MPTMS). The electrode was then incubated with 11-mercapunclecanoic acid (11-MIJA) and the nanoparticles were activated to allow for coupling to cyt c. This process resulted in the formation of the AuNP/cyt c hybrid on the ITO electrode. The ITO/AuNP/cyt c substrate surfaces were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffraction analysis (XRD), and cyclic voltammetry (CV) techniques. Further analysis regarding the surface roughness properties of ITO, ITO/AuNP and ITO/AuNP/cyt c were also performed. The ITO/AuNP/cyt c immobilized ITO electrode displayed a pair of well-defined redox peaks (E-pa at 0.09 V and E-pc at 0.02V) at pH 7.0 in HEPES buffer solution. Differential pulse voltammetry (DPV) and amperometric i-t measurements on the modified electrode showed a linear response after the addition of hydrogen peroxide (H2O2). The developed electrode sensor had an electron transfer rate constant (k(s)) of 0.69 s(-1) with a detection limit of 0.5 mu M. The results of this study suggest that the hybrid layers were well fabricated on the ITO surface and the developed ITO/AuNP/cyt c electrode displayed an excellent electrocatalytic response for the detection of H2O2. (C) 2011 Elsevier B.V. All rights reserved.
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