Galangin induces apoptosis in gastric cancer cells via regulation of ubiquitin carboxy-terminal hydrolase isozyme L1 and glutathione S-transferase P
- Authors
- Kim, Deuk Ae; Jeon, Young Keul; Nam, Myeong Jin
- Issue Date
- Mar-2012
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Flavonoid; Galangin; Gastric cancer; Apoptosis; Ubiquitin carboxy-terminal hydrolase; isozyme L1; Glutathione S-transferase P
- Citation
- FOOD AND CHEMICAL TOXICOLOGY, v.50, no.3-4, pp.684 - 688
- Journal Title
- FOOD AND CHEMICAL TOXICOLOGY
- Volume
- 50
- Number
- 3-4
- Start Page
- 684
- End Page
- 688
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16567
- DOI
- 10.1016/j.fct.2011.11.039
- ISSN
- 0278-6915
- Abstract
- Galangin has been shown to have anti-cancer property against several types of cancer cells. Many studies have described the anti-oxidant and apoptotic effects of galangin. However, the mechanism of galangin-induced apoptosis has not yet been studied for human gastric cancer cells. We investigated galangin-induced apoptosis of human gastric cancer SNU-484 cells. Galangin inhibited proliferation of SNU-484 cells in a dose- and time-dependent manner. The results showed that galangin significantly decreased the viability of SNU-484 cells at 50-200 mu M for 24 h and 48 h. Galangin-induced cell death was characterized with the changes in cell morphology. DNA fragmentation, cell cycle, activation of caspase-3/-9, poly (ADP-ribose) polymerase (PARP) cleavage, and expression of MAP kinase such as ERK1/2 and JNK. For identification of proteins potentially involved in apoptosis, a two-dimensional electrophoresis was employed. Proteomic analysis showed that several proteins were associated with anti-cancer properties of galangin. Of particular interest, these proteins included ubiquitin carboxy-terminal hydrolase isozyme L1 (Uch-L1) and glutathione S-transferase P (GSTP), which are involved in apoptosis of SNU-484 cells. Western blot analysis confirmed up-regulation of Uch-L1 and down-regulation of GSTP following galangin treatment. Our results suggest that Uch-L1 and GSTP be involved in galangin-induced apoptosis in human gastric cancer SNU-484 cells. (c) 2011 Elsevier Ltd. All rights reserved.
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