Apoptosis by aloe-emodin is mediated through down-regulation of calpain-2 and ubiquitin-protein ligase E3A in human hepatoma Huh-7 cells
- Authors
- Jeon, Won; Jeon, Young Keul; Nam, Myeong Jin
- Issue Date
- Feb-2012
- Publisher
- PORTLAND PRESS LTD
- Keywords
- aloe emodin; apoptosis; calpain-2; hepatocellular carcinoma; Huh-7 cells; ubiquitin-protein ligase E3A
- Citation
- CELL BIOLOGY INTERNATIONAL, v.36, no.2, pp.163 - 167
- Journal Title
- CELL BIOLOGY INTERNATIONAL
- Volume
- 36
- Number
- 2
- Start Page
- 163
- End Page
- 167
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16608
- DOI
- 10.1042/CBI20100723
- ISSN
- 1065-6995
- Abstract
- Natural flavonoids are associated with anti-proliferation of cancer growth. However, the antioxidant and anti-proliferation effects of AE (aloe-emodin) have not been well studied. We have investigated how AE affects the proliferation of hepatic hepatocellular carcinoma cells and exerts an anti-cancer effect. The cytotoxic effect of AE was demonstrated using an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay and Huh-7 cells were inhibited by AE treatment in both dose- and time-dependent manners. The IC50 level of AE was similar to 75 mu M. AE also has anti-proliferative effects via induction of DNA damage and apoptosis. 2-DE (two-dimensional electrophoresis) revealed that several proteins were related to the anti-cancer effects of AE. CAPN2 (calpain-2) and UBE3A (ubiquitin-protein ligase E3A), which are associated with the apoptosis signalling pathway, were verified by Western blotting. AE exhibited potent anti-proliferative effects on Huh-7 cells via down-regulation of CAPN2 and UBE3A. The findings support the possibility of AE being a chemopreventative agent.
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