동충하초가 파골세포의 분화와 유전자 발현에 미치는 영향Effects of Cordyceps militaris(CM) on Osteoclastogenesis and Gene Expression
- Other Titles
- Effects of Cordyceps militaris(CM) on Osteoclastogenesis and Gene Expression
- Authors
- 최경희; 유정은; 황귀서; 유동열
- Issue Date
- 2012
- Publisher
- 대한한방부인과학회
- Keywords
- Cordyceps militaris; Osteoclast; Osteoporosis; Osteoclastogenesis
- Citation
- 대한한방부인과학회지, v.25, no.3, pp.16 - 26
- Journal Title
- 대한한방부인과학회지
- Volume
- 25
- Number
- 3
- Start Page
- 16
- End Page
- 26
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16744
- ISSN
- 1229-4292
- Abstract
- Objectives: This study was performed to evaluate effects of Cordyceps militaris(CM) on osteoclast differentiation and its related gene expression.
Methods: We used mouse myeloid cells RAW 264.7 stimulated by receptor activator of nuclear factor kappa-B ligand(RANKL) to induce osteoclast differentiation.
There are four groups of which RAW 264.7 cells are not stimulated by RANKL (Normal), stimulated by RANKL without CM(Control), stimulated by RANKL with 0.1 ㎍/㎖ of CM(CM 0.1), stimulated by RANKL with 1 ㎍/㎖ of CM(CM 1).
Osteoclastogenesis was measured by counting Tartrate-resistant acid phosphatase-positive multinucleated cells [TRAP(+) MNC]. RT-PCR was performed to evaluate the inhibitory effect of CM on gene expression(TRAP, AKT1, JNK1, NFATc1, c-Fos,MITF).
Results:1. CM decreased the number of TRAP(+) osteoclast in RANKL-stimulated RAW 264.7 cell at the concentration of 0.1 ㎍/㎖ and 1 ㎍/㎖.
2. CM decreased the expression of TRAP in osteoclast at the concentration of 1 ㎍/㎖.
3. CM decreased the expression of AKT1, JNK1 in osteoclast at the concentration of 1 ㎍/㎖.
4. CM didn’t affect the expression of NFATc1, c-Fos, MITF in osteoclast.
Conclusions: Cordyceps militaris has inhibitory effects on osteoclast differentiation and its related gene expression. These results suggest that Cordyceps militaris has a potential as a treatment of osteoporosis.
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