어성초(魚腥草)의 면역활성에 미치는 영향

Abstract

Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages. Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide(H2O2) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal. Results:1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL. 3. After 24 hr incubation, HW increased significantly IL-1β production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL. 5. After 24 hr incubation, HW increased significantly TNF-α production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ㎍/mL. Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-1β, IL-6, and TNF-α in macrophages.