어성초(魚腥草)의 면역활성에 미치는 영향Immune Enhancing Effect of Houttuyniae Herba on Mouse Macrophage
- Other Titles
- Immune Enhancing Effect of Houttuyniae Herba on Mouse Macrophage
- Authors
- 김정현; 김윤상; 임은미
- Issue Date
- 2012
- Publisher
- 대한한방부인과학회
- Keywords
- Houttuyniae Herba; macrophage; cytokine; hydrogen peroxide; NO
- Citation
- 대한한방부인과학회지, v.25, no.2, pp.12 - 22
- Journal Title
- 대한한방부인과학회지
- Volume
- 25
- Number
- 2
- Start Page
- 12
- End Page
- 22
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17345
- ISSN
- 1229-4292
- Abstract
- Objectives: The aim of this study is to investigate immune enhancing effect of Houttuyniae Herba water extract(HW) on RAW 264.7 cell of mouse macrophages.
Methods: Effects of HW on productions of nitric oxide(NO) and hydrogen peroxide(H2O2) in RAW 264.7 mouse macrophages were measured. Effect of HW on production of cytokines such as interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α in RAW 264.7 cells was accessed by a multiplex bead array assay based on xMAP technology. All of results were represented P<0.05 compared to the normal.
Results:1. After 24 hr incubation, HW increased significantly NO production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL.
2. After 24 hr incubation, HW increased significantly hydrogen peroxide production in RAW 264.7 cells at the concentrations of 25, 50, 100 and 200 ㎍/mL.
3. After 24 hr incubation, HW increased significantly IL-1β production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL.
4. After 24 hr incubation, HW increased significantly IL-6 production in RAW 264.7 cells at the concentrations of 100 and 200 ㎍/mL.
5. After 24 hr incubation, HW increased significantly TNF-α production in RAW 264.7 cells at the concentrations of 50, 100, and 200 ㎍/mL.
Conclusions: These results suggest that HW has immune enhancing activity related with its increasement of NO, hydrogen peroxide, IL-1β, IL-6, and TNF-α in macrophages.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.