Quantitative phosphoproteomics of the human neural stem cell differentiation into oligodendrocyte by mass spectrometry
- Authors
- Cho, K.; Kim, J.Y.; Kim, E.; Wook Park, G.; Wook Kang, T.; Hae Yoon, J.; Kim, S.U.; Byun, K.; Lee, B.; Yoo, J.S.
- Issue Date
- Dec-2012
- Publisher
- 사단법인 한국질량분석학회
- Keywords
- LTQ-FT MS; Neural stem cell (NSC); Oligodendrocyte; Phosphoproteomics; SILAC; TiO2
- Citation
- Mass Spectrometry Letters, v.3, no.4, pp.93 - 100
- Journal Title
- Mass Spectrometry Letters
- Volume
- 3
- Number
- 4
- Start Page
- 93
- End Page
- 100
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17496
- DOI
- 10.5478/MSL.2012.3.4.93
- ISSN
- 2233-4203
- Abstract
- Cellular processes such as proliferation, differentiation, and adaptation to environmental changes are regulated by protein phosphorylation. In order to enhance the understanding of molecular dynamics for biological process in detail, it is necessary to develop sensitive and comprehensive analytical methods for the determination of protein phosphorylation. Neural stem cells hold great promise for neural repair following an injury or disease. In this study, we made differentiated oligodendrocytes from human neural stem cells using over-expression of olig2 gene. We confirmed using quantitative phosphoproteome analysis approach that combines stable isotope labeling by amino acids in cell culture (SILAC) and TiO2 micro-column for phosphopep-tide enrichment with MS2 and MS3 mass spectrometry. We detected 275 phosphopeptides which were modulated at least 2-fold between human neural stem cells and oligodendrocytes. Among them, 23 phosphoproteins were up-regulated in oligodendro-cytes and 79 phosphoproteins were up-regulated in F3 cells.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 의과대학 > 의예과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17496)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.