Protective Effect of Panaxynol Isolated from Panax vietnamensis against Cisplatin-Induced Renal Damage: In Vitro and In Vivo Studies
- Authors
- Lee, Dahae; Lee, Jaemin; Vu-Huynh, Kim Long; Thi Hong Van Le; Thi Hong Tuoi; Hwang, Gwi Seo; Park, Jeong Hill; Kang, Ki Sung; Minh Duc Nguyen; Yamabe, Noriko
- Issue Date
- Dec-2019
- Publisher
- MDPI
- Keywords
- Panax vietnamensis; panaxynol; cisplatin-induced renal damage; reno-protective activity; cytotoxicity; MAPKs; caspase-3
- Citation
- BIOMOLECULES, v.9, no.12
- Journal Title
- BIOMOLECULES
- Volume
- 9
- Number
- 12
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17933
- DOI
- 10.3390/biom9120890
- ISSN
- 2218-273X
- Abstract
- Polyacetylenic compounds isolated from Panax species are comprised of non-polar C17 compounds, exhibiting anti-inflammatory, antitumor, and antifungal activities. Panaxynol represents the major component of the essential oils of ginseng. We investigated whether panaxynol isolated from Panax vietnamensis (Vietnamese ginseng, VG) could prevent cisplatin-induced renal damage induced in vitro and in vivo. Cisplatin-induced apoptotic cell death was observed by staining with annexin V conjugated with Alexa Fluor 488, and western blotting evaluated the molecular mechanism. Panaxynol at concentrations above 0.25 mu M prevented cisplatin-induced LLC-PK1 porcine renal proximal tubular cell death. LLC-PK1 cells treated with cisplatin demonstrated an increase in apoptotic cell death, whereas pretreatment with 2 and 4 mu M panaxynol decreased this effect. Cisplatin demonstrated a marked increase in the phosphorylation of c-Jun N-terminal kinase (JNK), P38, and cleaved caspase-3. However, pretreatment with 2 and 4 mu M panaxynol reversed the upregulated phosphorylation of JNK, P38, and the expression of cleaved caspase-3. We confirmed that the protective effect of panaxynol isolated from P. vietnamensis in LLC-PK1 cells was at least partially mediated by reducing the cisplatin-induced apoptotic damage. In the animal study, panaxynol treatment ameliorated body weight loss and blood renal function markers and downregulated the mRNA expression of inflammatory mediators.
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